Aria 8.0 manuály

Aria 8.0 Uživatelský manuál (16 strany)

Značka: Aria | Kategorie: Tablety | Velikost: 1.50 MB |

Angličtina

Tabulka s obsahem

Flow Cytometry Core Facility

1

Prepared by Marc Barnard

1

Table of Contents

1

Introduction

2

Starting up the system

4

Optimizing the side streams

6

Aiming the streams

7

Page 10

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Page 11

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Page 12

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Page 13

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Page 14

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Page 15

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Aria 8.0 Uživatelský manuál (23 strany)

Značka: Aria | Kategorie: Tablety | Velikost: 1.02 MB |

Rumunština

Tabulka s obsahem

Manual de utilizare

1

Evolio Aria 8.0

1

Măsuri de protecţie

2

Cuprins

5

Accesorii

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Butoane şi porturi

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Încărcarea bateriei

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Pornirea unităţii

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Ecranul de start

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Aplicaţii preinstalate

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Deblocarea ecranului

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Accesul la Internet

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Lansarea browserului de Web

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Biblioteca de fişiere audio

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Vizualizarea fotografiilor

15

Navigarea în fişiere

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Setarea contului de email

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Gestionarea conturilor

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Ieşirea HDMI

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Gestionarea operaţiunilor

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Depanare

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Specificaţii

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Aria 8.0 Uživatelská příručka (344 strany)

Značka: Aria | Kategorie: Tablety | Velikost: 11.23 MB |

Angličtina

Tabulka s obsahem

BD FACSAria II

1

User’s Guide

1

FCC Information

3

Contents

5

About This Guide

13

Conventions

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Technical Assistance

15

Limitations

16

Cytometer Components

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Fluidics Cart

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Power and Operation

21

Flow Cytometer

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Fluidics Components

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Sample Injection Chamber

25

Tube Holders

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Cuvette Flow Cell

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Figure 1-11 Nozzle

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Sort Block

29

Deflection Plates

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Aspirator Drawer

30

Aerosol Management

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Sort Collection Chamber

32

Optics System

33

Collection Optics

35

Detectors

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Stream-Viewing Optics

38

Cytometer Electronics

39

Emergency Stop Button

40

Workstation

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Theory of Operation

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Fluid Movement

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Sample Flow

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Hydrodynamic Focusing

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Signal Generation

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Fluorescent Signals

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Signal Detection

50

Longpass Filters

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Bandpass Filters

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% transmission

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BP 500/50 filter

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DF 500/50 filter

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Neutral Density Filters

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Electronic Processing

57

Pulse Parameters

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Laser Delay

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Drop Formation

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Breakoff Window

62

Side Stream Formation

65

Drop Delay Overview

67

Drop Charging

68

Yield Mask

70

Purity Mask

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Phase Mask

72

Sort Precision Modes

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Defining New Precision Modes

75

Using BD FACSDiva Software

77

Workspace Components

78

Cytometer Controls

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Change Sample Filter

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Cleaning Modes

80

Sheath Pressure

80

Sample Agitation

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Sample Temperature

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Fluidics Level Indicators

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Cytometer Configuration

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Cytometer Status Report

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Custom Configurations

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Copying a Base Configuration

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Select the Sort Setup that

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Configuration Mismatch Dialog

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Acquisition Controls

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Sorting Controls

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Sort Menu

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Sort Setup

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Sort Layout

103

Setting Up a Sort Layout

105

Editing a Sort Layout

107

Using Sorting Controls

107

Using Counters

108

Monitoring a Sort

109

Sort Report

110

Templates

112

Running Samples

113

Cytometer Startup

114

Performing Fluidics Startup

115

Starting the Stream

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Setting Up the Breakoff

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Setting Up the Fluidics Cart

122

Refilling the Sheath Tank

123

ATTENTION

124

Emptying the Waste Container

127

Waste (A)

129

Preparing the CS&T Beads

133

Running a Performance Check

134

Reviewing the Results

134

Application Settings

136

Adjusting Area Scaling

137

Figure 4-14 FSC and SSC Plot

138

Optimizing PMT Voltages

141

Saving Application Settings

143

Data Collection

144

Calculating Compensation

148

Data Recording and Analysis

151

Setting Up the Experiment

152

Recording Data

155

Analyzing Data

156

Performing a Batch Analysis

159

Setting Up for Sorting

162

Setting Up for Bulk Sorting

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Using Manual Drop Delay

168

Overview of Auto Drop Delay

171

Using Auto Drop Delay

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Pausing and Resuming a Sort

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Setting Up the Stream

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Test Sort button

182

Creating a Custom Device

184

Deleting a Custom Device

186

Shutdown and Maintenance

187

Daily Shutdown

188

Fluidics Shutdown

190

External Cleaning

193

Scheduled Maintenance

194

Sample Line Backflush

195

Prime After Tank Refill

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Prepare for Aseptic Sort

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DI water port

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DI water line

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Purging the Fluid Filters

200

Purging the Sheath Filter

200

Changing Fluid Filters

202

Changing the Sheath Filter

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Changing the Sample Lines

204

Figure 6-12 Ferrule tool

207

Pinch Valve End

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Flow Cell End

209

Changing the Air Filters

211

Unscheduled Maintenance

213

Changing Nozzles

214

Cleaning the Nozzle

216

Cleaning the Camera Windows

225

Upper Camera Window

226

Apply lubricant to outside

229

Using Custom Optical Filters

230

Cleaning the Optical Filters

231

FSC ND filter

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Set screw

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Nozzle holder

232

Troubleshooting

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Troubleshooting the Stream

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Troubleshooting the Breakoff

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Sorting Troubleshooting

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Acquisition Troubleshooting

244

Fluidics Troubleshooting

251

Electronics Troubleshooting

252

Technical Specifications

253

Cytometer Specifications

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Performance

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Sort Performance

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Excitation Optics

257

Emission Optics

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Detector Arrays

258

Fluidics Cart Specifications

259

Appendix A

261

Supplies and Consumables

261

Cytometer Supplies

262

Accessory Kit

264

Other Replacement Parts

266

Consumables

267

Reagents

268

Appendix B

271

Near UV Laser Option

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System Laser Configurations

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Specifications

273

Operation

274

Instrument Setup

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Experiment Setup Tips

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Appendix C

281

BD Aerosol Management Option

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Option Components

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ULPA Filter

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Starting Up the Evacuator

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Maintenance

289

Replacing the ULPA Filter

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Reset button

293

Replacing the Air Filter

294

Appendix D

301

Temperature Control Option

301

Setting Up the Water Bath

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Cooling OutCooling In

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Setting Up the Tube Holder

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Input tubing

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Output tubing

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Setting Up the ACDU Stage

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Starting Up the Water Bath

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Appendix E

313

QC Using BD FACSDiva Software

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Software

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Preparing QC Particles

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Setting Up the QC Experiment

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Recording QC Data

325

Figure E-6 Recorded QC data

327

Reusing the QC Experiment

328

Tracking QC Results

329

Numerics

331

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